Feature Talk - The crucial role of CHI3L1 in Vasculogenic Mimicry formation of Cervical Cancer

Abstract:

Statement of the Problem: Over the past several decades, accumulating evidence has revealed that highly metastatic cancers are intimately associated with a vessel-like formation that is primarily derived from tumor cells, independent of endothelial cell-mediated angiogenesis. This alternative microvascular formation lacking endothelial cells is known as vasculogenic mimicry (VM). VM develops tumor vascular networks that associated tumor growth, metastasis, and short survival time of cancer patients. However, the knowledge of VM in the vascularization of cervical cancer are not fully understood yet. Chitinase-3-like-1 (CHI3L1) has been reported to plays a critical role in angiogenesis of cervical cancer. Here, we explored a pathological function of CHI3L1 in tumor cell-mediated vascularization. Methodology & Theoretical Orientation: The sixty-six tissue samples of cervical cancer were collected to determine CHI3L1 expression and VM formation using immunohistochemistry and CD34-periodic acid-Schiff (PAS) dual staining. Findings: CHI3L1 expression was significantly correlated with VM formation (p = 0.031). Interestingly, patients with VM positive tumors tended to have decreased overall survival (OS) compared to those with VM negative samples (43.9 versus 64.6 months, p = 0.079). In addition, recombinant CHI3L1 enhanced cervical cancer cell lines to form tube-like structures, supporting the notion that CHI3L1 mediates VM in cervical cancer. Conclusion & Significance: Our present findings suggest the crucial role of CHI3L1 by promoting the formation, which may contribute to tumor aggressiveness. Therefore, CHI3L1 may represent a novel attractive therapeutic target for the reduction of cervical cancer vascularization and metastasis.

Speaker Biography:

Nipaporn Ngernyuang has completed her Ph.D. in Biomedical sciences from Khon Khan University, Thailand. Currently, she is an Assistant Professor at Chulabhorn International College of Medicine, Thammasat University, Thailand. Her program of research focuses on molecular oncology and nanotechnology for cancer treatment. She has published about 8 papers in reputed journals. She has received researcher awards for her scholarly work from Thammasat University.

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Poster Presentation - Influence of NECC (Nutrition and Exercise Center for Cancer Patients) on survival and body composition in Gastrointestinal Cancer Patients

Abstract BACKGROUND : Gastrointestinal cancer patients are particularly predisposed to develop cancer cachexia. Cachectic patients are characterized by loss of adipose tissue as well as skeletal muscle wasting which has a tremendous impact on quality of life and survival (1, 4). In terms of improvement for this multifactorial syndrome, Klinikum rechts der Isar (MRI) in Munich, Germany has founded NECC, a treatment concept including nutrition intervention and an individual training program. METHODS : In this study we focused on patients with pancreatic (n =15) and upper gastrointestinal cancer (n = 21). Body composition using CT images was analyzed initially, 4 weeks, 3 months and 6 months after initial visit of NECC (Image 1). To underline the outcome, NECC patients were matched to adequate partners who correspond in age, gender, diagnosis with TNM classification and type of surgery. The control group does not receive any further support concerning nutrition and physical exercise

Conference Venue

holiday Inn Rome - Aurelia,Via Aurelia Km, 8400 00165, Rome, Italy About City Rome is the capital city of Italy and Lazio region with 2,872,800 residents in 1,285 km2. It is the fourth most populous city in the European Union by population within city limits. Rome is located in central western Italian peninsulas. Rome has acquired the status of a global city. In 2016, Rome has been ranked as the 14th most visited cities in the world and 3rd most visited cities in Europe. It is a city that is proud of its ancient glorious heritage, a city that once expanded its empire throughout Europe, Africa and Asia. The city of Rome includes tourist places such as Colosseum, Pantheon, Spanish Steps, the Forum, Trevi Fountain, and the catacombs any of the buildings have arches, domes and vaults. Colosseum is one of the famous architecture symbols in Rome which is capable of housing 60,000 spectators and it is the largest amphitheater ever built in the Roman empire and it was used for gladia

Poster Presentation - The Notch ligand DLL1 exerts pro-carcinogenic effects in human breast cancer luminal A MCF-7 cells

Abstract Background: Breast cancer (BC) is the most common cancer in women and has a high rate of relapse and death. The Notch signaling pathway plays an important role in normal breast development and homeostasis. Dysregulation of Notch receptors and its ligands Jagged1, Jagged2, and DLL4 has been detected in BC and implicated in tumor development, progression, drug resistance, and recurrence. The Notch ligand DLL1 has emerged as a player in BC as its expression is undetectable in normal breast tissues, but moderate to high in BC. In this study, we examined the role of DLL1 in BC luminal A MCF-7 cells. Methodology: DLL1 siRNA and recombinant DLL1 protein were used to evaluate the effects of DLL1 in MCF-7 cells. Gene expression was analyzed by qRT-PCR and immunoblotting. Cell growth and proliferation were assessed by trypan blue exclusion and MTT methods. Microscopy and scratch wound-healing assays were used to evaluate colony formation and cell migration. Findings: In M

25th Global Meet on Cancer Research & Oncology

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